diff --git a/Editor/AOT/Analyzer.cs b/Editor/AOT/Analyzer.cs
index 68253e3..dcd2a87 100644
--- a/Editor/AOT/Analyzer.cs
+++ b/Editor/AOT/Analyzer.cs
@@ -17,12 +17,16 @@ namespace HybridCLR.Editor.AOT
             public AssemblyReferenceDeepCollector Collector { get; set; }
 
             public int MaxIterationCount { get; set; }
+
+            public bool ComputeAotAssembly { get; set; }
         }
 
         private readonly int _maxInterationCount;
 
         private readonly AssemblyReferenceDeepCollector _assemblyCollector;
 
+        private readonly bool _computeAotAssembly;
+
         private readonly HashSet<GenericClass> _genericTypes = new HashSet<GenericClass>();
         private readonly HashSet<GenericMethod> _genericMethods = new HashSet<GenericMethod>();
 
@@ -47,6 +51,7 @@ namespace HybridCLR.Editor.AOT
         {
             _assemblyCollector = options.Collector;
             _maxInterationCount = options.MaxIterationCount;
+            _computeAotAssembly = options.ComputeAotAssembly;
             _methodReferenceAnalyzer = new MethodReferenceAnalyzer(this.OnNewMethod);
             _hotUpdateAssemblyFiles = new HashSet<string>(options.Collector.GetRootAssemblyNames().Select(assName => assName + ".dll"));
         }
@@ -71,7 +76,7 @@ namespace HybridCLR.Editor.AOT
 
         private bool IsAotType(TypeDef type)
         {
-            return !_hotUpdateAssemblyFiles.Contains(type.Module.Name);
+            return _computeAotAssembly || !_hotUpdateAssemblyFiles.Contains(type.Module.Name);
         }
 
         private bool IsAotGenericMethod(MethodDef method)
diff --git a/Editor/Commands/AOTReferenceGeneratorCommand.cs b/Editor/Commands/AOTReferenceGeneratorCommand.cs
index c2d6340..b4dfb41 100644
--- a/Editor/Commands/AOTReferenceGeneratorCommand.cs
+++ b/Editor/Commands/AOTReferenceGeneratorCommand.cs
@@ -2,6 +2,7 @@
 using HybridCLR.Editor.Meta;
 using System;
 using System.Collections.Generic;
+using System.IO;
 using System.Linq;
 using System.Text;
 using System.Threading.Tasks;
@@ -22,6 +23,10 @@ namespace HybridCLR.Editor.Commands
             GenerateAOTGenericReference(target);
         }
 
+        /// <summary>
+        /// 计算热更代码中的泛型引用
+        /// </summary>
+        /// <param name="target"></param>
         public static void GenerateAOTGenericReference(BuildTarget target)
         {
             var gs = SettingsUtil.HybridCLRSettings;
@@ -40,5 +45,88 @@ namespace HybridCLR.Editor.Commands
             writer.Write(analyzer.AotGenericTypes.ToList(), analyzer.AotGenericMethods.ToList(), $"{Application.dataPath}/{gs.outputAOTGenericReferenceFile}");
             AssetDatabase.Refresh();
         }
+
+
+
+        //[MenuItem("HybridCLR/Generate/AOTGenericReference2", priority = 103)]
+        //public static void GeneratedAOTGenericReferenceExcludeExists()
+        //{
+        //    GeneratedAOTGenericReferenceExcludeExists(EditorUserBuildSettings.activeBuildTarget);
+        //}
+
+        /// <summary>
+        /// 计算热更新代码中的泛型引用，但排除AOT已经存在的泛型引用
+        /// </summary>
+        /// <param name="target"></param>
+        /// 
+        public static void GeneratedAOTGenericReferenceExcludeExistsAOTClassAndMethods(BuildTarget target)
+        {
+
+            var gs = SettingsUtil.HybridCLRSettings;
+            List<string> hotUpdateDllNames = SettingsUtil.HotUpdateAssemblyNamesExcludePreserved;
+
+            AssemblyReferenceDeepCollector hotUpdateCollector = new AssemblyReferenceDeepCollector(MetaUtil.CreateHotUpdateAndAOTAssemblyResolver(target, hotUpdateDllNames), hotUpdateDllNames);
+            var hotUpdateAnalyzer = new Analyzer(new Analyzer.Options
+            {
+                MaxIterationCount = Math.Min(10, gs.maxGenericReferenceIteration),
+                Collector = hotUpdateCollector,
+            });
+
+            hotUpdateAnalyzer.Run();
+
+
+            string aotDllDir = SettingsUtil.GetAssembliesPostIl2CppStripDir(target);
+            List<string> aotAssemblyNames = Directory.Exists(aotDllDir) ?
+                Directory.GetFiles(aotDllDir, "*.dll", SearchOption.TopDirectoryOnly).Select(Path.GetFileNameWithoutExtension).ToList()
+                : new List<string>();
+            if (aotAssemblyNames.Count == 0)
+            {
+                throw new Exception($"no aot assembly found. please run `HybridCLR/Generate/All` or `HybridCLR/Generate/AotDlls` to generate aot dlls before runing `HybridCLR/Generate/AOTGenericReference`");
+            }
+            AssemblyReferenceDeepCollector aotCollector = new AssemblyReferenceDeepCollector(MetaUtil.CreateAOTAssemblyResolver(target), aotAssemblyNames);
+            var aotAnalyzer = new Analyzer(new Analyzer.Options
+            {
+                MaxIterationCount = Math.Min(10, gs.maxGenericReferenceIteration),
+                Collector = aotCollector,
+                ComputeAotAssembly = true,
+            });
+
+            aotAnalyzer.Run();
+
+            var (resultTypes, resultMethods) = ExcludeExistAOTGenericTypeAndMethodss(hotUpdateAnalyzer.AotGenericTypes.ToList(), hotUpdateAnalyzer.AotGenericMethods.ToList(), aotAnalyzer.AotGenericTypes.ToList(), aotAnalyzer.AotGenericMethods.ToList());
+            var writer = new GenericReferenceWriter();
+            writer.Write(resultTypes, resultMethods, $"{Application.dataPath}/{gs.outputAOTGenericReferenceFile}");
+            AssetDatabase.Refresh();
+        }
+
+
+        private static (List<GenericClass>, List<GenericMethod>) ExcludeExistAOTGenericTypeAndMethodss(List<GenericClass> hotUpdateTypes, List<GenericMethod> hotUpdateMethods, List<GenericClass> aotTypes, List<GenericMethod> aotMethods)
+        {
+            var types = new List<GenericClass>();
+
+            var typeSig2Type = hotUpdateTypes.ToDictionary(t => t.Type.DefinitionAssembly.Name + ":" + t.ToTypeSig(), t => t);
+            foreach (var t in aotTypes)
+            {
+                string key = t.Type.DefinitionAssembly.Name + ":" + t.ToTypeSig();
+                if (typeSig2Type.TryGetValue(key, out var removedType))
+                {
+                    typeSig2Type.Remove(key);
+                    Debug.Log($"remove AOT type:{removedType.ToTypeSig()} ");
+                }
+            }
+
+            var methodSig2Method = hotUpdateMethods.ToDictionary(m => m.Method.DeclaringType.DefinitionAssembly.Name + ":" + m.ToMethodSpec().ToString(), m => m);
+            foreach (var m in aotMethods)
+            {
+                string key = m.Method.DeclaringType.DefinitionAssembly.Name + ":" + m.ToMethodSpec().ToString();
+                if (methodSig2Method.TryGetValue(key, out var removedMethod))
+                {
+                    methodSig2Method.Remove(key);
+                    Debug.Log($"remove AOT method:{removedMethod.ToMethodSpec()} ");
+                }
+            }
+
+            return (typeSig2Type.Values.ToList(), methodSig2Method.Values.ToList());
+        }
     }
 }